我国鼠疫菌毒力因子的比较与分析

Comparison and analysis of the virulence factors of Y.pestis in China

目的 研究自不同生态型和不同鼠疫自然疫源地分离的鼠疫菌 4种毒力因子的性状特征。方法 常规方法检查鼠疫菌毒力因子性状并比较在不同疫源地之间、不同生态型之间的差异。结果 132 6株鼠疫菌中仅 1株缺失FⅠ、6株缺失PstⅠ ,Pgm和VW阳性分别为 92 99%、76 47%。结论 自我国不同疫源地、不同生态型和不同年代分离的野生型鼠疫菌绝大多数能产生FⅠ和PstⅠ ,且不同生态型和不同疫源地分离的菌株这两种毒力因子无明显差别 ,性状稳定。VW-和Pgm-菌株与生态型及疫源地有一定的关系。经长期人工培养传代后 ,4种毒力因子相比 ,VW因子较易丧失 。

Objective To study the characteristics of four virulence factors from various ecological types and isolated from different natural plague foci in China. Methods By examining the characteristics of virulence factors of Y.pestis : Fraction Ⅰ antigen (FⅠ), Pestitin (PstⅠ), Pigmentation (Pgm) and VW antigen (VW), compared the difference among ecological types and isolation foci. Results In all 1326 strains of Y.pestis , only 1 lacks FⅠ, 6 lack PstⅠ. The positive rates of Pgm and VW are 92.99% and 76.47%, respectively. Conclusions FⅠ and PstⅠ are identified in nearly all the wild Y.pestis isolated from various foci, with ecological types and in different time. The strains which lack Pgm and VW antigen are related to the ecological types and foci. After long term of transplantation in succession in artificial medium, VW antigen was unstable in all four virulence factors.