丙型肝炎病毒包膜2区准种复杂性及其临床意义初探

Genetic complexity of the envelope region 2 (E2) of hepatitis C virus (HCV) in HCV infected individuals

目的 研究丙型肝炎病毒 (HCV)包膜 2 (E2 )区准种复杂性及其临床意义。方法 以 9例不同血清丙氨酸转氨酶水平的急性HCV感染者和 4例慢性丙型肝炎患者为对象 ,通过逆转录PCR扩增HCVE2区氨基端片段 (5 6 0bp) ,扩增产物直接克隆 ,以单链构象多态性 (SSCP) /异质性双体 (HD)分析对每份血清标本的 30个克隆进行筛选及准种复杂性分析。结果 慢性丙型肝炎患者血清HCV准种复杂性明显增高 ;HCV感染者血清ALT水平变化与准种复杂性无明显关系。结论 HCVE2区准种复杂性与病毒持续性感染有关 。

Objective To study genetic complexity of HCV from HCV infected individuals and its clinical significance. Methods Acute phase plasma samples from 9 HCV infected individuals with different levels of alanine aminotransferase ( ALT) and persistent phase plasma samples from four patients with chronic hepatitis C were obtained. A 570bp fragment from the E2 region was amplified by reverse transcriptase PCR and cloned. Thirty cloned cDNAs representing each specimen were assessed by a method that combined a single stranded conformational polymorphism (SSCP) and heteroduplex analysis(HDA) method to determine the number of clonotypes (electrophoretically indistinguishable cloned cDNAs ) as a measure of quasispecies complexity. The results were discussed refering clinical features of infection. Results Quasispecies complexity was more evident in those with persistent viremia, but not correlated to ALT activity. Conclusion Genetic complexity of HCV E2 is associated with HCV persistence but not with the clinical severity of the disease.