脑肠肽在针刺调节免疫反应中的作用(英文)

Brain-gut peptides in electro-acupuncture-regulation of immunoreactions in rats

目的 研究电针 (EA)刺激大鼠足三里穴对神经 -内分泌 -免疫网络的影响及其机制 .方法 将大鼠随机分成正常对照组、足三里组、免疫抑制模型组、非经非穴组、足三里 +免疫抑制组 .应用放射免疫法测定脑垂体和外周血 P物质和血管活性肠肽含量的变化 ;应用流式细胞仪技术 ,通过微量全血直接免疫荧光染色法测定外周血 T细胞亚群以反映细胞免疫功能 ;应用红细胞 C3b受体 -酵母菌花环试验和红细胞 - IC花环试验检测红细胞免疫功能 .结果 电针刺激足三里穴后正常大鼠外周血的 CD4+ ,RBC- C3b RR,RBC- ICR均明显升高 (P<0 .0 1) ,CD8+无显著变化 (P>0 .0 5 ) ;脑垂体和外周血中 SP,VIP的含量增加 (P<0 .0 1) ;且 CD4+与 SP,VIP含量的变化 (r=0 .744 ,P<0 .0 5 ;r=0 .738,P<0 .0 5 ;r=0 .82 2 ,P<0 .0 5 ;r=0 .848,P<0 .0 5 ) ,CD4+ 和 RBC- C3b RR呈显著正相关 (r=0 .719,P<0 .0 5 ) .免疫抑制组大鼠 CD4+ ,RBC- C3bRR明显低于正常对照组 (P<0 .0 5 ) ,CD8+ 无显著变化 (P>0 .0 5 ) ;脑垂体和外周血中 SP,VIP的含量减少 (P<0 .0 1) .足三里 +免疫抑制组 CD4+ (P<0 .0 1) ,RBC- C3b RR(P<0 .0 1)以及脑垂体和外周血中 SP,VIP的含量 (P<0 .0 1)与免疫抑制组有差异 ,与正常对照组相比无显著差异 (P >0 .0 5 ) .

AIM To investigate the role of brain gut peptides in the electro acupuncture regulated neuroendocrine immune network as well as its mechanism. METHODS The rats were randomized into 5 groups, including the control, the Tsusanli , the immunosuppressive model, the non acupoint group, and the Tsusanli +immunosuppressive groups. The Tsusanli group rats, fixed, were electro acupunctured at Tsusanli (He Sea Point, St 36) on both sides continually for 30 min at any point between 07:00 and 09:00 for 7 d on a daily basis. The location of the acupoints for EA: Tsusanli (He Sea Point, St 36) was identified at approximately 5 mm below the small end of the fibula on the outer lower side of knee joint hind limb of the rats and the non acupoint was 0.5 cm beyond Tsusanli . Changes in substance P (SP) and vasoactive intestinal peptide (VIP) contents in the pituitary gland and peripheral blood were determined by radioimmunoassay. T lymphocytic subgroups were assayed to reflect the cellular immunity. Erythrocytic immunity was assayed by red blood cell C 3b receptor rosette (RBC C 3b RR) and red blood cell immune complexes rosette (RBC ICR). RESULTS After EA, the immuno adhesiveness of T lymphocytic subgroups and erythrocytes of both healthy rats and immuno suppressive rats was enhanced. What is more, the values of SP and VIP in rat pituitary gland and peripheral blood increased signifi cantly in comparison with that of the control and were positively correlated with the change in CD 4 +. There was no significant difference between the indexes of the non acupoint group and that of the control. CONCLUSION EA at Tsusanli can positively regulate the T lymphocytic immunity and erythrocytic immuno adhesiveness in the peripheral blood of both normal and immuno suppressive groups of rats. When the immune system is regulated by EA at Tsusanli , the contents of SP and VIP in the central and peripheral blood are positively correlated with the change in the immune system, indicating that SP and VIP have an important role in the EA regulation of the neuroendocrine immune network. The results of our study, consistent with previous findings in the field, make an important complement to the regulatory effects of EA on the immune system.