多西紫杉醇诱导骨肉瘤凋亡的实验研究

Docitaxol induction apoptosis of osteosarcoma

目的 研究多西紫杉醇对骨肉瘤细胞生长抑制及诱导凋亡作用。方法 应用细胞计数、形态学观察、蛋白含量测定、流式细胞术和电镜等方法对多西紫杉醇诱导的骨肉瘤进行检测和观察。结果 多西紫杉醇在浓度为 4μg/ml时 ,对骨肉瘤细胞有明显的生长抑制作用 ,并且存在着时间依赖关系和一定范围内的剂量依赖关系。多西紫杉醇可将骨肉瘤细胞阻断于G1期 ,并诱导凋亡 ,其凋亡细胞具有典型凋亡细胞特征。用流式细胞仪测定细胞周期 ,可见明显的凋亡峰。电镜观察染色质沿皱缩的核膜下凝聚 ,细胞表面出现凋亡小体 ,基质呈絮状致密化。在紫杉醇短时间作用去除后再培养比持续作用更易促使细胞凋亡。同时 ,多西紫杉醇对荷瘤小鼠也有明显的抑制作用。结论 多西紫杉醇对骨肉瘤细胞的细胞毒作用是诱导其凋亡的结果 ,这种诱导凋亡的能力是紫杉醇疗效的基础 ,在对骨肉瘤的治疗中 ,紫杉醇有着巨大的潜力。

Objective To study the growthinhibition and apoptosisinduction effect of docitaxol on osteosarcoma cells.Methods The effect of docitaxol on osteosarcomat cell line PL 1 was examined by cell count, morphologic observation, flow cytometry (FCM) and electronmicroscopey.Results Docitaxol inhibitied the growth of PL 1 in a time and dose dependent manner. The osteosarcoma cells were blocked at G1 phase of the cell cycle. The treated cells presented features typical of apoptosis. Cells recultureed shortly after being treated with docitaxol were more apt to undergo apoptosis than cells treated with docitaxol continuouslly.Conclusion The growth inhibiting effect of docitaxol is due to its ability to induce apopotisis. It shows great potential in the treatment of osteosarcoma.