两种黑色素瘤抗原基因在肝细胞癌中的表达

Expression of two types of melanoma antigens in hepatocellular carcinoma

目的 检测黑色素瘤抗原MAGE 4和MAGE 10mRNA在中国人肝细胞癌 (HCC)中的表达 ,为用这两种基因编码蛋白作为疫苗对HCC患者进行免疫治疗提供依据。方法 用RT PCR方法对 48例HCC患者癌组织和相应癌旁肝组织、10例肝硬化组织和 10例正常肝组织的MAGE 4和MAGE 10mRNA表达情况进行研究 ,并对RT PCR扩增产物中目的基因片段进行cDNA序列测定。结果 48例HCC患者中 ,有 15例 (31.3% )表达MAGE 4,14例 (2 9.2 % )表达MAGE 10mRNA ;而相应癌旁肝组织、肝硬化和正常肝组织均不表达。cDNA测序进一步证明 ,PCR产物中的目的基因片段为MAGE 4和MAGE 10cDNA序列 ,两种MAGE基因的表达与肿瘤大小、甲胎蛋白 (α FP)水平等临床指标无相关关系。结论 MAGE 4和MAGE 10在HCC组织中呈特异性的表达 ,而在癌旁非瘤性肝组织中均不表达 ,这使得以此抗原用于HCC患者的免疫治疗成为可能。

Objective To investigate the expression of melanoma antigen MAGE 4 and MAGE 10 mRNA in Chinese human hepatocellular carcinoma (HCC).Methods The expression of MAGE 4 and MAGE 10 mRNA in HCC tissues and the adjacent non HCC liver tissues was studied using RT PCR in 48 samples of HCC. Ten samples of cirrhosis and 10 samples of normal liver tissues were examined. The PCR products were sequenced.Results Of the 48 HCC samples studied, 15 (31.3%) and 14 (29.2%) expressed MAGE 4 and MAGE 10 mRNA respectively. In contrast, none of the HCC adjacent non tumorous liver tissues were MAGE 4 and MAGE 10 mRNA detectable. Nor did liver tissues from cirrhosis and normal liver samples. The expression of the two genes in HCC showed no correlation with the serum level of AFP and the tumor size.Conclusion MAGE 4 and MAGE 10 mRNA are specifically expressed in Chinese HCC samples.