bax基因在子宫内膜癌中的表达及意义

Bax gene expression in endometrial carcinoma

目的 研究bax基因在子宫内膜癌发生发展中的作用。方法 采用RT PCR方法检测 8例正常增殖期子宫内膜、6例子宫内膜单纯及复合增生、6例子宫内膜不典型增生及 42例子宫内膜癌组织中bax基因mRNA的表达。结果 以 8例正常增殖期子宫内膜为表达参照 ,在 1例子宫内膜复合增生、2例子宫内膜非典型增生和 2 7例 (6 4.3% )子宫内膜癌中 ,bax基因mRNA过表达 ,其余为中度表达。在子宫内膜癌中 ,随着临床分期及组织学分级的增加、肌层侵袭的加深以及淋巴结的转移 ,bax基因mRNA过表达阳性率逐渐增加 (除临床分期P >0 .0 5外 ,其余均P <0 .0 5 )。结论 bax基因参与子宫内膜细胞增殖与凋亡过程 ,与子宫内膜癌的发生发展有关 ,可作为评估子宫内膜癌高危因素和恶性生物学行为的重要参考指标之一。

Objective To study the relationship between bax gene expression and endometrial carcinoma. Methods Bax mRNA expression was evaluated by RT PCR in a series of 8 normal proliferative endometrium (NPE), 6 simple and complex hyperplasia (SCH), 6 atypical hyperplasia (AH) and 42 endometrial carcinoma (EC).Results All of the 8 NPE showed moderate bax mRNA expression. In 1 of 6 SCH, 2 of 6 AH and 27 of the 42 EC (64.3%), there was overexpresion of bax mRNA. In endometrial carcinoma, with the progression of clinical stage, increase in histologic grade and in depth of invasion to muscle layer, and lymph node metastersis,the positive rate of bax overexpression was correspondingly increased.Conclusion The results suggest that bax expression affects the balance between the endometrial cell proliferation and apoptosis. Bax might play an important role in carcinogenesis and tumor progression of endometrial carcinoma. As a high risk factor it can be used to evaluate the biological behavior of endometrial carcinoma.