nm23基因表达与乳腺癌远处转移及预后相关性的研究

Expression of nm23 in breast cancer:correlation with distant metastasis and prognosis

目的 探讨nm2 3基因表达与乳腺癌远处转移的关系及其预后意义。方法 选取两组共 16 9例乳腺癌患者 ,应用免疫组化和RT PCR方法检测nm2 3基因表达。结果 nm2 3基因表达与乳腺癌远处转移及腋淋巴结转移呈显著负相关 ,与生存率呈正相关。第 1组病例发生远处转移的 9例腋淋巴结阴性患者中 ,7例为nm2 3蛋白低表达 ;而 2 9例未发生远处转移的腋淋巴结阳性患者中 ,nm2 3蛋白中表达及高表达者为 2 4例 (82 .8% )。第 2组发生复发和远处转移的 6例患者其nm2 3mRNA均低表达。nm2 3基因表达与腋淋巴结转移、肿瘤大小均为影响预后的因素。结论 nm2 3基因作为一个独立的预后指标应用 ,可弥补常规指标的不足 ,具有重要的临床应用价值。

Objective To investigate the relationship between expression of nm23 gene and distant metastasis and prognosis in breast cancer. Methods To detect nm23 protein expression in 101 patients with breast cancer by means of immunohistochemistry and nm23 mRNA expression in 68 patients with breast cancer by RT PCR. Results Expression of nm23 gene was inversely correlated with distant metastasis and lymph node metastasis. Among 9 patients with negative node but with distant metastasis, 7 had low expression of nm23. In 29 patients with positive nodes but without distant metastasis, 24 had high expression of nm23, while 6 of 6 patients with positive nodes and distant metastasis expressed high levels of nm23 mRNA. Conclusion Expression of nm23 gene, tumor size and status of axillary lymph node are independent prognostic indictors to breast cancer prognosis.