应用6种M-H培养基测试磺胺类药物抑菌圈直径的结果分析

Analysis of assay results of antimicrobial zone diameters by sulfomides using six Mueller-Hinton media

目的对 6种 M- H培养基进行比较 ,选择测试磺胺类药物的最佳 Mueller- Hinton(M- H)培养基。方法按照 NCCL S的要求 ,采用纸片扩散法 ,在 6种 M- H培养基上用 3株标准菌株 :粪肠球菌 (ATCC2 92 12 ) ,大肠埃希菌 (ATCC2 5 92 2 ) ,金黄色葡萄球菌 (ATCC2 5 92 3)对 (SMZco)分别进行 5 0次测定。用 10株测试菌株在 6种 M- H培养基上对 SMZco分别进行 10次测定。结果美国 Difco公司和英国 Oxoid公司的 M- H培养基在用粪肠球菌ATCC2 92 12测试 SMZco抑菌圈直径时 ,5 0次抑菌圈直径测定值均大于 2 0 m m,其他四种 M- H培养基的测定值都小于 2 0 mm。用大肠埃希菌 (ATCC2 5 92 2 ) ,金黄色葡萄球菌 (ATCC2 5 92 3)测试 SMZco抑菌圈直径时 ,6种 M-H培养基的全部测定值都在 NCCL S规定的范围内。 10株测试菌株对 SMZco的抑菌圈直径测定 ,除 Difco公司和Oxoid公司 M- H培养基外 ,其他 4种 M- H培养基均存在不同程度的双环现象。结论 Difco公司和 Oxoid公司 M-H培养基合格 ,其他

Objective Six M H media were compared for selecting the best M H in the sulfomides antimicrobialtest. Methods According to NCCLS, paper disks diffusion method were adopetd, 3 standard strains for E. faecalis(ATCC 229212), E.coli(ATCC 25922) and S. aureus(ATCC 25923) each were used to test SMZco 50 times on six M H media;10 test strains each were used to test SMZco 10 times on six M H media. Results 50 times inhibitory zone diameters>20 mm when E. faecalis(ATCC 29212) were tested to SMZco by using Difco and Oxoid company M H media, 50 times inhibitory zone diameters<20 mm when other four M H media were used. All test results in NCCLS limits when using E.coli (ATCC 25922) and S.aureus(ATCC 25923) to test SMZco on six M H media. When 10 test strains were used to test SMZco zone diameters, other four M H media possess different degree double ring phenomena except for Difco and Oxoid company M H media. Conclusion Difco and Oxoid company M H media are qualified, other four M H media are not qualified.