协同凝集试验检测O1群霍乱弧菌

Coagglutination test for detecting V. Cholerae serotype O1

目的建立一种快速简便的 O1群霍乱弧菌检测方法。方法将抗 O1群霍乱弧菌多价单克隆抗体包被到金黄色葡萄球菌 Cowan I株上制成 SPA菌体试剂 ,建立起协同凝集试验 (COA) ,检测粪便标本 6 h培养物中的霍乱弧菌。结果 COA检测的敏感性为 4× 10 6 CFU/ m l,检测过程为 5 min。 92份临床标本的测定结果与培养法相比较 ,COA的特异性、敏感性、诊断符合率分别是 10 0 %、95 .5 %、97.8%。结论 COA简便、快速 。

Objective To develop a simple and rapid method for detecting V. cholerae O1. Methods Staphylococcus aureus Cowan I was coated with anti V. cholerae O1 monoclonal antibodies and a coagglutination test(COA) detecting V. cholerae O1 in 6 h fecal enrichment cultures was tested. Results The detect limit of COA was 4×10 6 CFU/ml, and detect time was 5 min. When 92 samples were tested, the diagnostic specificity, sensitivity and accuracy of the test, compared with standard culture methods, were 100%、95 5%、97 8%,respectively. Conclusion The COA is simple and less time consuming, and can be used as a new method for rapid detection of V. Cholerae.