^(60)Co源意外受照者红细胞血型糖蛋白A变异频率

Variant Frequency of Erythrocyte Glycophorin A in Persons Accidentally Irradiated by ^(60)Co Source

血型糖蛋白A(glycophorinA ,GPA)是体细胞突变研究的重要分子标志之一。为了了解射线作用与GPA突变的效应关系 ,采用GPA突变分析技术对 3例钴源意外受照个体外周血红细胞GPA变异频率进行了检测。经淋巴细胞染色体畸变分析方法进行估算 ,这 3例个体受照的生物剂量分别为 2 .5 ,2 .9和 1.9Gy ,所测GPA变异细胞频率分别是正常对照个体均值的 3.9,4 .3和4 .1倍 ,提示射线所致GPA变异细胞频率增高效应。受照个体外周血中长期持续存在的GPA变异细胞 ,来源于突变的造血干细胞 。

Glycophorin A(GPA) is one of the important molecular markers in studies of somatic cell mutations. To investigate the relationship between the γ irradiation and the frequency of GPA variation, the frequency of variant erythrocytes at the GPA locus was determined in peripheral blood of 3 subjects with accidental whole body γ irradiation. The biological dose of individuals was 2.5, 2.9 and 1.9 Gy estimated by the chromosome aberration assay, respectively, and the frequency of GPA variation was 3.9, 4.3 and 4.1 times greater than that from normal controls, respectively. Our results suggest that the variant frequency of erythrocyte GPA was increased. On account of the GPA gene mutations are preserved in hematopoietic stem cells during all irradiated individual′s life, the frequency of GPA variation could be used as a permanent marker for mutagenesis of radiation.