一氧化氮与氧化修饰低密度脂蛋白在肾病时的变化及其临床意义

Changes of Nitric Oxide and Oxidatively Modified Low Density Jipoprotein in Nephropathy and Their Clinical Significance

目的 探讨NO及ox LDL在肾病时的变化及其与肾功能的关系。方法 采用分光光度法和酶联免疫吸附法分别测定了 48例各类肾病血中的NO和ox LDL水平。结果 各类肾病患者血中NO和ox LDL的水平均明显高于正常对照组 (NO为 (0 .6 6± 0 .18) μmol/L ,ox LDL为 (0 .32± 0 .0 7)mg/L) ;NO随疾病的发展而逐渐降低 ,与病程长短成反比 (r= - 0 .30 19) ;ox LDL则随疾病的进展而逐渐升高 ,与病程长短呈正相关 (r =0 .410 2 ) ,二者在肾病时呈明显负相关 (r =- 0 .7186 )。结论 肾病时机体过多地产生NO ,也存在脂质过氧化程度增强 ,NO极有可能参与了肾病的发生、发展过程 ,ox LDL则可能是肾病发生发展的始动因素 ,也是并发心脑血管疾病的危险因素。

Objective To study the changes and relationships of nitric oxide (NO) and oxidatively modified low density lipoprotein (ox-LDL) in nephropathy. Method Spectrophotometry and exzyme linked immunosorbent assay (ELISA) were used to test the levels of NO and ox-LDL in serum or plasma of 49 patients with nephropathy. Result The NO and ox-LDL from 28 patients with primary glomerular, 21 with secondary glomerular were (1.34±0.25)μmol/L and (0.47±0.13)mg/L, (1.02±0.23)μmol/L and (0.69±0.18)mg/L respectively. The NO and ox-LDL levels were significantly higher than in controls [(0.66±0.18)μmol/L and (0.32±0.07)mg/L]. The NO levels fell down with the progress of diseases (r=-0.301 9), while ox-LDL levels inceased (r=0.410 2), and the correlation coefficient between NO and ox-LDL was -0.718 6. Conclusion There was more NO in nephropathy which participated in the progress of nephropathy. The lipid peroxidation increased, and ox-LDL was not only the first factor in nephropathy, but the risky factor in cardivascular and cerebrovascular diseases.