新疆6种蚊虫的rDNA-ITS2区序列分析

Sequences analysis of the ribosomal DNA second internal transcribed spacer for six mosquito species in Xinjiang, China

测定并分析了新疆 ４种伊蚊和 ２种按蚊的 ｒＤＮＡ—ＩＴＳ２区序列，新疆伊蚊、刺扰伊蚊ＩＴＳ２区长度分别为 ２３１ｂｐ～２５６ ｂｐ、２５０ｂｐ或 ２５３ｂｐ，其克隆间、种内序列差异分别为 ０．３％～４．３％、０．８％～１．４％；里梅伊蚊与长刀伊蚊的长度为３３０ ｂｐ、２５１ ｂｐ，种内序列无差异；４种伊蚊种间序列差异明显大于种内差异水平，为１２．３％～３３．５％；赫坎按蚊和米赛按蚊的 ＩＴＳ２区序列长度分别为 ４６３ｂｐ、３１１ｂｐ，种内个体间无差异，而种间的差异为 ３０．８％．结果显示 ｒＤＮＡ－ＩＴＳ２区序列差异，为形态上易混淆蚊种提供了有意义的分子鉴别特征。

The ribosomal DNA second internal transcribed spacer of four aedine and two anopheline mosquitoes in Xinjiang was sequenced and analysed. The length and intraspecific variation of rD- NA-ITS2 region were 231bp^256bp, 0.3%~4.3% in Aedes sinkiangensis, 250bp or 253bp, 0.8% ~1.4% in Aedes vexans, respectively. The length was 330bp in Aedes caspius, 251bp in Aedes longifilamentus, and the sequences among clones and individuals were the same. The level of interspecific variation among four species of aedine mosquitoes was 12.3%~33.5%, more than the intraspecific. The ITS2 region of Anopheles hyrcanus and An. messeae were 463bp and 311bp in length, 30.8% in sequence variation between them. The sequence differences of rDNA-ITS2 region were the foundation of molecular identification for confusable species.