摘要
通过采用盐析和透析等提纯方法 ,对解脂假丝酵母 (Candidalipolytica)脂肪酶进行了纯化 ,比活提高了 2 .69倍 ,回收率 45% .该酶反应的最适pH值为 7.5,最适温度为 38℃ ,pH稳定范围为 6.0～ 7.5,温度稳定性较差 ,以 2mol/L的蔗糖作保护剂效果较好 .该酶为金属酶 。
By means of salting out and dialysis, the lipase from Candida lipolytica was purified with the results of the specific activity 2.69 times higher and the activity recovery of 45%. The property research shows that the optimum pH and temperature of crude enzyme were 7.5 and 38 ℃ respectively and that the stabilization range of pH was 6.0～7.5. With lower temperature stabilization, it was found that the 2 mol/L saccharose was better protectant than others. Being metalloenzyme, the lipase has better activation in presence of 5 mmol/L calcium ion.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2001年第3期257-260,共4页
Journal of Wuxi University of Light Industry
基金
江苏省科委应用基础项目! (BJ990 37)
教育部高等学校骨干教师资助计划项目!资助课题
关键词
解脂假丝酵母
脂肪酶
纯化
稳定性
Candida lipolytica
lipase
purification
stabilization
