河北省肠出血性大肠杆菌O157∶H7感染监测

Surveillance of E.coli O157∶H7 Infection in Hebei Province

为调查河北省既往是否曾发生肠出血性大肠杆菌O15 7∶H7感染疑似病例 ,了解动物带菌和市售食品、饮水的污染情况。选取市级以上医院 ,查阅 1998年 1月 1日至 1999年 12月 31日的住院病案 ,对有腹泻史的急性肾功能衰竭患者填写个案调查表。采集各种动物粪便、市售食品和饮水标本 ,接种山梨醇 -麦康凯培养基 ,可疑菌落转种三糖铁培养基 ,之后进行生化鉴定和血清学鉴定。结果表明 ,全省共发现 41例肠出血性大肠杆菌O15 7∶H7感染疑似病例 ,分布于 8个市 ,发病时间无严格季节性。男性 2 9例 ,女性 12例 ;年龄最小者 8天 ,最大者 78岁 ;农民所占比例最大 ,占 6 8 3%。全省采集十几种动物粪便标本共计 2 0 6 0份 ,从牛粪 (75 1份 )中分离到 2株O15 7∶H7;采集五类市售食品共 35 2 3份 ,从生猪肉 (36 6份 )中分离到 2株O15 7∶H7;采集的饮水标本未分离到O15 7∶H7。因此证实河北省存在发生肠出血性大肠杆菌O15

Objective To investigate whether probable cases of E.coliO157∶H7 infection were occurred in Hebei province, and to obtain surveillance information on the prevalence of E.coli O157∶H7 in animal hosts, food products and drinking water. Methods Municipal or provincial hospitals were selected. Cases of acute renal failure with diarrhea history was discovered through consulting the case histories of in-patients who entered the hospital between Junuary 1, 1998 and October 31, 1999, and a questionnaire was administered. Faeces of animal hosts, food products and drinking water were collected and inoculated on sorbitol MacConkey agar. Suspected colonies were inoculated in triple sugar iron agar, and then did the biochemical and serological identification. Results 41 probable cases of E.coli O157∶H7 infection distributed in 8 cities were discovered. There was no rigid seasonality in the onsets of the illness. Those probable cases were aged 8 days to 78 years, 29 male and 12 female. 68.3% were farmers 2060 samples of the faeces of more than 10 species animals were collected, and 2 strains of E.coli O157∶H7 was isolated from faeces of cattle. Several kinds of food products were also collected, and 2 strains of E.coli O157∶H7 were isolated from raw pork. No strain of E.coli O157∶H7 were isolated from the drinking water. Conclusion There was a potential risk of outbreak of E.coli O157∶H7 infection in Hebei province.