苯乙烯对青年男性工人遗传毒性的研究

Study on the Genetic Toxicity of Styrene to Exposed Young Male Workers

[目的 ]研究苯乙烯对青年男性工人的遗传毒性。 [方法 ]根据苯乙烯的平均浓度设高、中、低三个剂量组 ,测其苯乙烯代谢产物尿中苯乙醛酸 (PGA)、苯乙醇酸 (MA)浓度 ,以外周血淋巴细胞的染色体畸变率及微核率作为遗传毒性指标 ,并与其有关因素进行相关回归分析。 [结果 ]高剂量组染色体畸变率高于对照组 (P <0 0 5 ) ;微核率的结果则是 :当苯乙烯浓度为 5 7 2mg/m3 时 ,或班后尿中PGA +MA浓度为 0 442 g/L± 0 0 5 6g/L时 ,微核率高于对照组 (P <0 0 5 ) ,且随苯乙烯浓度的增高而增加。微核率及染色体畸变率与苯乙烯平均浓度、班后尿中MA +PGA浓度、工龄和吸烟量呈正相关 ,与饮酒和年龄无相关。 [结论 ]苯乙烯对接触男工具有遗传毒性 ,且呈一定的剂量—效应关系。班后尿中MA +PGA浓度可以考虑作为苯乙烯接触的生物监测指标。

Objective] To study on the genetic toxicity of styrene to young male workers [Methods] Workers exposed to styrene were classified into three groups(high, middle and low dose) by the average density of styrene To detect the metabolites (phenylglyoxylic acid,PGA and mandelic acid MA) of styrene in the urine The rate of chromosome aberration (CA) and micronucleus (MN) of lymphocytes were counted as the indexes of genetic toxicity Then the relative factors were analyzed by stepwise regression [Results] The CA rate in high dose group was higher than control group (P<0 05);When the concentration of styrene was 57 2 mg/m 3 or the concentration of PGA+MA in urine was 0 442 g/L±0 056 g/L , there was significantly different in MN rate The MN rate increased with the concentration styrene The MN and CA rates were positive correlation with the concentration of styrene and PGA+MA in urine, working age and smoking But they did not correlate with alcohol drinking and age [Conclusion] The genetic damage of styrene can be found in the exposed youth male workers and there exist the dose effect relationship PGA+MA in urine can be considered a biologic monitoring index of styrene