cyclin D1、p53蛋白及DNA倍体在评价肺癌预后中的作用

Role of cyclin D1 protein, p53 protein and DNA ploidy in judging malignancy and prognosis of lung cancer

目的 探讨细胞周期素蛋白D1(cyclinD1)、p5 3蛋白、DNA倍体模式在肺癌的恶性程度及预后判断中的作用。方法 采用流式细胞术 (flowcytometry ,Fcm)方法检测 40例肺癌组织、15例癌旁正常肺组织中的cyclinD1、p5 3蛋白的表达情况及细胞核DNA倍体模式。结果 肺癌中cyclinD1、p5 3蛋白表达阳性率分别为 62 .5 % ( 2 5 / 40 )、75 % ( 30 / 40 ) ,表达量明显高于癌旁正常肺组织 (P <0 .0 1) ,异倍体率为 77.5 % ( 31/ 40 ) ,cyclinD1、p5 3蛋白过表达及DNA倍体与组织学类型、病理分级、淋巴结转移、存活时间有关。结论 cyclinD1蛋白、p5 3蛋白、DNA倍体在评价肺癌的恶性程度及预后上有重要意义。

Objective To study the role of cyclin D1, p53 Protein and DNA ploidy in judging the degree of malignancy and prognosis of lung cancer. Methods Paraffin-embedded specimens from 40 lung cancer patients and 15 paracancerous normal lung tissues were examined by flow cytometry. Results The positive rate of cyclin D1, p53 protein and aneuploidy was 62.5%(25/40), 75%(30/40) and 77.5%(31/40) respectively. The levels of cyclin D1 protein and p53 protein in lung cancer tissues were significantly higher than those in normal lung tissues (P<0.01). Overexpression of cyclin D1, p53 protein and aneuploidy was related with tissue types, pathological grade, lymphatic metastasis and survival time. Conclusion Cyclin D1 protein and p53 protein play an important role in carcinogenesis of the lung. It is valuable to determine cyclin D1, p53 protein and aneuploidy for judging malignancy and prognosis of lung cancer patients.