冬小麦旗叶蔗糖和籽粒淀粉合成动态及与其有关的酶活性的研究

Studies on the Dynamic Changes of the Synthesis of Sucrose in the Flag Leaf and Starch in the Grain and Related Enzymes of High-yielding Wheat

鲁麦 2 2和鲁麦 14相比 ,旗叶的光合能力强 ,蔗糖含量高 ,催化蔗糖合成的磷酸蔗糖合成酶(SPS)活性高 ;籽粒中蔗糖供应充足 ,降解蔗糖的蔗糖合成酶 (SS)活性高 ;与淀粉合成有关的酶 :腺苷二磷酸葡萄糖焦磷酸化酶 (ADPG- PPase)、游离态 (或可溶性 )淀粉合成酶 (S- STS)和束缚态淀粉合成酶 (B- STS)均有较高的活性 ,这是其粒重高的原因。通过对两个品种各个处理有关淀粉合成的酶活性与淀粉积累速率和灌浆速率的相关分析表明 ,SS、ADPG- PPase、S- STS、B- STS的活性均与淀粉积累速率和籽粒灌浆速率呈极显著正相关关系 ,说明这

The synthesis of sucrose in the flag leaves of Lumai 22 and Lumai 14, a high yielding and an ordinary winter wheat respectively, and the accumulation of starch in their grains were studied at regular intervals. The results indicated that the pattern of the dynamic changes of the syntheses of sucrose and accumulation of starch was quite similar to that of the activities of their related enzymes. It also revealed that the starch accumulating rate and grain filling rate were highly significantly correlated with the activities of their related enzymes, including SS, ADPG PPase, S STS and B STS. Comparison of the two cutivars showed that Lumai 22 always had higher sucrose content, higher starch content, higher synthetic rate and accumulation rate, and higher enzymes activities than Lumai 14. All these suggested that these superior characteristics might be the reason of the higher kernel weight of Lumai 22 and these four enzymes were key enzymes that effect the synthesis rate of starch.