摘要
成功地建立了检测兔出血症病毒 (RHDV)抗原的金银染色 (SECGA)三抗体夹心法 ,并确定了试验流程中鼠抗RHDV -IgG(1∶1 0 )、兔抗RHDV -IgG(1∶1 60 )、金标羊抗兔IgG(1∶50 )等三项的最佳工作浓度及最适显影时间 (2 0min) .实验结果表明 ,本法除了具有Dot-ELISA的高度特异性外 ,还具有抗原量少、敏感性高、不参与 3,3-二氨基联苯胺盐酸盐等有害物质、实验结果更可靠等优点 ,适合于现场推广应用 ,并为兔出血症的诊断提供可靠的实验手段 .
A double sandwich gold-siler stain assay was developed for the detection of Rabbit Hemorrhagic Disease Verus(RHDV) antigen.The optimum working concentration of mouse anti-RHDV immunoglobulin G(IgG) was determined to be 1∶10、that of rabbit anti-RHDV IgG to be 1∶160、gold-linked goat anti rabbit IgG to be 1∶5q0 and the optimal time for the reaction with the developer to be 20 minutes.The results showed that besides the high specificity of Dot ELISA,this method has the virtues of little use of antigen,high sensitivity and reliability.It can serve as a reliable laboratory method for the diagnosis of RHDV.
出处
《延边大学农学学报》
2001年第2期88-92,共5页
Agricultural Science Journal of Yanbian University
