水稻与空心莲子草原生质体电融合条件的研究

Study on the Electrofusion Condition of Protoplasts between Rice and Alternanthera Philoxeroides

以水稻 (OryzasativaL . )的胚性悬浮细胞和空心莲子草 [Alternantheraphiloxeroides(Mart.)Griseb ]的悬浮细胞为材料 ,研究了两种原生质体电融合的条件。结果表明 :在交流电场为 10 0V/cm ,直流脉冲电场为 1.5kV/cm ,脉冲幅宽为 4 0 μs ,脉冲间隔为 0 .5s ,脉冲次数为 2次 ,电融合液组成为 10 %甘露醇和 0 .4mmol/LCaCl2 时 ,原生质体的融合频率达到 2 4 .6 %。

The study was attempted to find the condition suitable for the electrofusion of protoplasts isolated from cell suspension cultures of Oryza sativa L. and Alternanthera philoxeroides (Mart.) Griseb. It showed that the fusion frequency of protoplasts reached 24.6% with the following parameters: alternate current (AC) 100V/cm , direct current (DC) 1.5kV/cm , DC pulse width (PW) 40μs , DC pulse interval 0.5 s, Number of DC pulse 2 , 10% mannitol and 0.4 mmol/L CaCl2 in electrofusion solution.