鸡IL-15基因的分子克隆及其有关特性的研究

Cloning and Characterization of Chicken Interleukin-15 Gene

实验应用聚合酶链式反应技术从鸡脾淋巴细胞中克隆得到了白细胞介素_15基因 ,序列分析表明与已发表的鸡白细胞介素_15基因完全一致。核苷酸序列和推导的氨基酸序列与牛的最接近 ,同源性分别为 46 %和 31%。与哺乳动物白细胞介素_15序列类似 ,有 4个高度保守的半胱氨酸残基。同时本研究也用此方法对鸡脾脏、法氏囊、胸腺、哈德氏腺和盲肠扁桃体等淋巴器官的淋巴细胞中鸡白细胞介素_15mRNA的表达进行了研究 ,结果表明这些器官的淋巴细胞均表达mRNA。本实验还用有丝分裂原ConA对鸡脾淋巴细胞进行活化 ,观察活化的淋巴细胞表达白细胞介素_15mRNA的情况 。

A cDNA encoding chicken interleukin_15(ChIL_15)was cloned from chicken splenocytes by reverse transcription_polymerase chain reaction(RT_PCR)method.The chicken IL_15 cDNA contains an open reading frame encoding 143 amino acid.Nucleotide and amino acid sequence analyses of chicken IL_15 revealed 46% and 31% identity with bovine IL_15 respectively.RT_PCR also demonstrated that chicken IL_15 gene is expressed in many lymphatic organs including thymus,spleen,bursa of Fabucicius,Harder's gland and caecum tonsil.The results were also indicated that the activation of spleen cells with ConA could enhance the expression of IL_15 mRNA.