摘要
采用田间育性鉴定和RFLP分子标记的方法对玉米C型胞质不育的恢复基因进行了研究 .田间育性结果表明 ,玉米C型胞质不育有两对重叠恢复基因 ,通过对A192近等基因系和 (CMS C2 37×A6 19)BC1分离群体的RFLP分子标记 ,将玉米的C型胞质不育恢复基因Rf4定位在第 8染色体短臂上 ,与RFLP探针npi2 2 0和csu2
A study was conducted on the restoring gene( Rf4 )of C cytoplasmic male sterility in maize by means of field fertility determination and RFLP molecular marker.Feild fertility results show that there were two pairs of overlapping restoring genes of C cytoplasmic male sterility in maize.Using the method of near isogenic line A192 and the BC 1 segregating population of (CMS C237×A619) restriction fragment length polymorphism(RFLP),the restoring gene Rf4 for C cytoplasmic male sterility was localized in chromosome 8 S,linking to two RFLP markers loci.npi220 and csu29.
出处
《河南农业大学学报》
CAS
CSCD
2001年第2期99-102,共4页
Journal of Henan Agricultural University
基金
国家自然科学基金!资助项目 ( 3 9470 45 1)
