摘要
采用不同制片方法对蜡梅 (Chimonanthuspraecox)根尖进行染色处理 ,结果表明 ,用质量浓度为 2g·L-1的秋水仙碱预处理根尖 ,体积分数为 45 %的醋酸离解 ,醋酸—洋红染色效果较好 .采用这种方法 ,在 15× 40倍的显微镜下可观察到 11对染色体 .
Chimonanthus praecox root tip was stained with different methods. The results indicated that the staining effect was better under the conditions of pre treating the root tip with 2 g·L -1 colchicines, disrupting by 45% acetic acid and staining with acetic acid magenta. With this method, eleven pairs of Chromosome were observed under the microscope(multiple 15×40).
出处
《河南农业大学学报》
CAS
CSCD
2001年第2期140-141,148,共3页
Journal of Henan Agricultural University
关键词
蜡梅
根尖
染色体
制片
Chimonanthus praecox
root tip
chromosome