HPLC分离甘肃棘豆中苦马豆素

Isolation and Identification of Swainsonine from O. Kansuensis Bunge

甲醇浸泡甘肃棘豆粉 ,回收甲醇浸泡液至浸膏 ,1 mol/L HCl溶解后上 73 2型强酸性阳离子交换柱 ,1 mol/L NH4 OH洗脱并挥发至干 ,得到总生物碱。总生物碱经氨性氯仿提取后 ,通过高效液相色谱柱分离 ,色谱条件是 :反相 ( C1 8硅胶 )柱 ,在 2 5min内用 5%～ 1 0 0 %甲醇线性梯度洗脱 ,苦马豆素一般出现在 80 %～1 0 0 %的范围内 ,表现为一个宽峰。高效液相色谱柱分离后回收溶液得到一种白色细针状结晶 1 .0 4 mg,植物中的提取率为 0 .0 0 52 %。经 TLC、MP、IR、MS鉴定分析 。

O.kansuensis Bunge powder was dipped in methanol. The residue following evaporation of the methanol was dissolved in 1 mol/L HCl and applied to a strong cation exchange resin and eluted with 1 mol/L NH 4OH. After extracting the crude alkaloid with ammoniated chloroform, the residue was preparation by HPLC, using reverse phase (C 18 silica) and water/methanol, starting at 5% methanol and a linear gradient to 100% in 25 minutes. 1.04 mg crystal was obtained by HPLC, the yield was 0.0052% based on the plant. The crystal was swainsonine identified by TLC, MP, IR and MS.