人类巨细胞病毒感染对人胚肺细胞HOXB1,HOXB5,HOXB6及HOXB9基因表达的影响

Influence of human cytomegalovirus infection on the expressions of HOXB1, HOXB5,HOXB6,and HOXB9 genes in human embryo lung cells

目的 :研究人胚肺 (HEL)细胞HOXB1,HOXB5 ,HOXB6及HOXB9基因的表达状态及人类巨细胞病毒(HCMV)感染对上述基因表达的影响。方法 :采用半定量逆转录 -聚合酶链反应 (RT -PCR)技术。结果 :①HEL细胞表达HOXB5和HOXB6 ,但不表达HOXB1和HOXB9;②HCMV感染后 ,HEL细胞HOXB6基因的表达上调 ,且被HCMV诱导表达HOXB9T ,而HOXB5基因的表达无明显改变 ,HOXB1基因仍旧不表达 ;③全反式维甲酸 (ATRA)能上调HCMV感染后HOXB9基因的表达 ,但对HCMV感染晚期HOXB6基因的表达有抑制作用。结论 :HCMV能诱导HOXB6和HOXB9基因表达异常 。

Objective To study the expressions of HOXB1, HOXB5,HOXB6, and HOXB9 genes in human embryo lung (HEL) cells and influence of human cytomegalovirus (HCMV)infection on the expressions of these genes. Method The expressions of HOXB1, HOXB5, HOXB6, and HOXB9 genes were detected with semi-quantitative RT-PCR method. Results ① HEL cells expressed HOXB5 and HOXB6 genes, but they did not express HOXB1 and HOXB9 genes. ②After HCMV infection, HEL cell was induced to express HOXB9 gene, as the expression of HOXB6 gene was elevated. The expression of HOXB5 gene had no significant change. HOXB1 was still not expressed. ③Treated with all-trans retinoic acid (ATRA), the expression of HOXB9 gene in HEL cells infected by HCMV was significantly increased. But during the advanced infect period, the expression of HOXB6 was significantly decreased. Conclusion HCMV can induce abnormal expressions of HOXB6 and HOXB9 genes, which may play an important role in HCMV-induced abnormal embryogenesis.