锌和维生素E对肝辐射损伤治疗保护作用的实验研究

Experimental research of protective and therapeutic effects of zinc and vitamin E on mouse liver radiational damage

观察小鼠肝辐射损伤后锌 (Zn)和维生素E(VE)对肝匀浆中脂质过氧化物 (LPO)含量和谷胱甘肽过氧化物酶 (GSH Px)活力的影响。采用60 Coγ射线一次性全身照射 (7 5Gy)小鼠 0 5h后给予Zn和VE 进行不同时间的保护性治疗 ,测定肝组织匀浆中LPO含量和GSH Px活力。结果发现 ,60 Co照射小鼠后 ,其肝组织匀浆中LPO值显著升高(P <0 .0 1) ,GSH Px活力明显下降 (P <0 .0 1)。单纯Zn治疗组 ,其LPO值无明显下降 ,而GSH Px活力明显升高 (P<0 .0 1)。单纯VE 组 ,LPO值明显下降 (P <0 .0 5 ) ,GSH Px活力显著增加 (P <0 .0 1)。Zn +VE 联合治疗组与单纯VE组比较 ,在治疗后第 15d ,GSH Px活力显著增加 (P <0 .0 1)。提示在肝辐射损伤中 ,补充VE 对降低LPO含量、增加GSH Px活力均有重要作用。Zn +VE 联合治疗在提高GSH Px活力、阻止肝细胞脂质过氧化。

Objective To study the effects of zinc and vitamin E on lipid peroxide (LPO) contents and glutathione peroxidase (GSH Px) activities in liver homogenates of radiational damage mice. Methods Two hundred and forty mice were divided randomly into five groups: normal control group (Group A), 60 Co γ ray irradiator group (Group B), 60 Co+zinc (Zn) group (Group C), 60 Co+vitamin E(V E) group (Group D), and 60 Co+Zn+V E group (Group E). After irradiated with 60 Co γ ray 7.5 Gy, the mice were protectively treated with Zn and V E at different times. LPO contents and GSH Px activities in liver homogenates of the mice were determined. Results The level of liver LPO was significantly higher (P<0.01), but the level of liver GSH Px was significantly lower (P<0.01) in Group B than those in Group A; the content of liver GSH Px was markedly higher in Group C and Group D than that in Group B (P<0.01), while the content of liver LPO was markedly lower in Group D than that in Group B (P<0.05); the concentration of liver GSH Px was markedly higher in Group E than that in Group D (P<0.01). Conclusions V E may play an important role in lowering liver LPO contents and raising liver GSH Px activities in radiational damage mice. vitamin E can cooperate with zinc in raising liver GSH Px activities, stopping liver cells from lipids peroxidation and keeping integrity of liver cell membrane.