Bcl-2和bax蛋白在大鼠脑缺血预处理脑组织中的表达

Bcl 2 and baxexpression in rat cerebral ischemic preconditioning

目的 :探讨凋亡相关基因bcl 2和bax蛋白在脑缺血预处理过程中的表达及意义。方法 :用免疫组织化学SP法分别对假手术对照组、缺血预处理对照组、缺血组和缺血预处理组大鼠前脑组织冰冻切片进行检测。结果 :缺血预处理对照组bcl 2蛋白的表达高于其他 3组 ,而各组bax蛋白表达差异不显著。结论 :缺血预处理对bcl

Objective To study the expression of apoptosis regulated genes bcl 2 and bax nocoprotein expression and its significance in rat cerebral ischemic preconditioning. Methods With immunohistochemical assay, bcl 2 and baxexpression were studied in rat brains which were divided into four groups: the sham-operation control group, the cerebral ischemic group and the cerebral ischemic preconditioning group. Results Bcl 2 oncoprotein was induced in cerebral ischemic preconditioning control group. Bax oncoprotein expression had no obviously difference in four groups. Conclusions The expression of bcl 2 and bax oncoprotein after sublethal ischemic preconditioning might be associated with the preconditioning protection against neuronal damage following subsequent lethal ischemia.