神经元GABA合成、分解和分泌与癫痫发病的研究

Metabolism and Secretion of GABA in Kindle hippocampus neurons

目的 研究神经元的γ-氨基丁酸 (GABA)合成、分解和分泌与癫痫发病的关系。方法 用致痫剂马桑内酯 (CL )和抗痫剂苯妥英钠 (PHT)处理培养大鼠神经元 ,用高效液相色谱仪 ,紫外分光光度计分别检测培养神经元内外的 GABA浓度和神经元内γ-氨基丁酸转氨酶 (GABA - T)活性。结果 与对照组比较 ,CL使神经元内GABA- T活性显著增强 (P<0 .0 5 ) ,对神经元内外 GABA浓度没有影响。与 CL处理组以 (10 0μmol/ L CL培养神经元 2 4h)比较 ,PHT使 CL致痫神经元内 GABA- T活性显著减弱 (P<0 .0 5 ) ,基本恢复到对照组水平。但神经元内外 GABA浓度仍相对稳定。结论 癫痫发病中 ,痫性神经元合成、分解 GABA增强 ,分泌

Objective To study relationship between γ aminobutyric acid(GABA) and epilepsy. Methods Coriaria lactone (CL) and phenytoin sodium(PHT) were used to treate hippocampus neurons in rats. The levels of GABA and activity of GABA transaminase(GABA T) were measured by high performance liquid chromatograph(HPLC) and ultraviolet spectrophotometer. Results The activity of intracellular GABA T in CL treated neurons was significantly higher than that in control group (P<0.05). The activity of GABA T in CL treated neurons was significantly decreased when pretreated with PHT(P<0.05). The levels of extracellular and intracellular GABA had no statistic difference between CL treated and PHT treated neurons. Conclusion At the period epilepsy attack,GABA in kindle neurons synthesis and decompose increases.