黄芪对糖尿病性肾病大鼠心肌脂质过氧化作用及一氧化氮水平的影响

THE EFFECT OF ASTRAGALUS MEMBRANACEUS(FISCH) BGE. ON LIPID PEROXIDATION AND NO LEVEL OF MYOCARDIUM FROM RATS WITH DIABETIC NEPHROPATHY

目的 :研究黄芪对糖尿病性肾病大鼠心肌脂质过氧化作用及NO水平的影响。方法 :雄性Wistar大鼠分为正常对照组、糖尿病组、糖尿病性肾病组、糖尿病性肾病伴急性肾功能损害组、黄芪治疗组。分别测定各组心肌组织内SOD、GSH -Px、NOS活性及MDA、NO含量。结果 :各实验组心肌组织内GSH -Px、SOD活性下降 ,NOS活性及MDA、NO含量增高 ,其中以糖尿病性肾病伴急性肾功能损害组最明显 ,黄芪对之有纠正作用。结论 :黄芪能抑制糖尿病性肾病心肌脂质过氧作用并降低NO水平 。

Aim: To study the effect of astragalus membranaceus(Fisch)bge. on the lipid peroxidation and NO level of myocardium from rats with diabetic nephropathly.Methods: Male wistar rats were divided into five groups: ① Aormal control group,② Diabetes group,③ Diabetic nephropathy group, ④ Diabetic nephropathy group complicated with acute renal function damage,⑤ Astragalus membranaceus(Fisch)bge. treatment group. The activity of superoxide dismutase(SOD), glutathione peroxidase(GSH Px), nitric oxide synthase(NOS) and content of malondialdehyde (MDA), nitric oxide(NO) was determined biochemically from myocardial homogenate among different groups.Results: The activity of GSH Px, SOD decreased and the content of MDA, NO and NOS activity increased in each experimental group compared with control group, as was more obvious in diabetic nephropathy group with acute renal function damage, which astragalus membranaceus(Fisch)bge could improve. Conclusion: astragalus membranaceus(Fisch)bge. could protect the myocardium in diabetic nephropathy by inhibiting lipid peroxidation and decreasing the level of NO.