生长激素和催乳素放射免疫测定法的建立与应用

THE RADIOIMMUNOASSAY OF GROWTH HORMONE AND PROLACTIN AND ITS APPLICATION

目的 :建立测定大鼠垂体和血浆中生长激素 (GH)和催乳素 (PRL)含量的高特异性、高灵敏度的双抗放射免疫测定 (RIA)法 ;研究急性低氧对大鼠垂体激素GH和PRL的作用。方法 :用氯胺 T法进行抗原放射性碘标记 ;采用平衡饱和加样程序的双抗RIA法测定。结果 :用该方法测定急性低氧 (0 .5h)时血浆和垂体GH和PRL含量 ,7km低氧 ,垂体GH含量明显升高 (P <0 .0 5 ) ,血浆则相反 :7km低氧 ,明显降低垂体和血浆PRL含量 (P <0 .0 1) ;而 5km低氧对GH和PRL的作用与对照相比无统计学差异。结论 :本双抗RIA法具有高特异性、高灵敏度及简便易行等特性 ;用该法测定提示急性低氧可抑制大鼠GH和PRL的分泌。

Aim:To set up a highly specific and sensitive double antibody radioimmunoassay(RIA)for determining the contents of growth hormone (GH) and prolactin(PRL) in pituitary and plasma of rats;To study the effects of acute hypoxia on GH and PRL of rats.Methods:We use chloramine T technique iodinate antigen and choose the equilibratory and saturated procedure of RIA to add the preparations.Results:Exposed to 7 km hypoxia for 0.5 h,pituitary GH content increased ( P <0.05) and conversely, plasma GH levels were suppressed( P <0.05);while the PRL levels in both pituitary and plasma were suppressed ( P <0.01);and there was no significantly difference between 5 km hypoxia groups and control.Conclusion:We have successfully established the method of radiolabel and double antibody RIA with highly sensitivity;acute hypoxia (7 km) suppressed the secretion of GH and PRL in rats.