壳多糖拮抗TNF促进兔主动脉内皮细胞与单核细胞粘附作用

Inhibitory Effects of Chitosan on TNF Induced Adhesion Reaction of Rabbit Aortic Endothelial Cells With Monocytes

目的：研究肿瘤坏死因子（ＴＮＦ）对体外培养的免主动脉内皮细胞（ＲＡＥＣ）与单核细胞（ＭＣ）粘附的影响及壳多糖的拮抗作用。方法：通过测定ＭＣ髓过氧化物酶活性的方法，对粘附于ＲＡＥＣ单层上的ＭＣ进行定量。结果：ＴＮＦ在５－５００Ｕ·ｍｌ－１时诱导ＲＡＥＣ与ＭＣ的粘附，ＴＮＦ浓度为２５０Ｕ·ｍｌ－１时促粘附达最大值（８１．９８％）。ＴＮＦ与内皮细胞共育１ｈ，即可明显促进粘附作用（６６．８％），４ｈ时达最大刺激效应（７７．５％）。壳多糖在浓度为２０－２０００ｍｇ·Ｌ－１时，呈剂量依赖性地拮抗ＴＮＦ诱导的粘附作用，在２０００ｍｇ·Ｌ－１时抑制率达２２．９９％。结论：壳多糖具有抑制由ＴＮＦ诱导的ＲＡＥＣ与ＭＣ粘附的作用。

OBJECTIVE.. To study inhibitory effects of chitosan on tumor necrosis factor(TNF)induced adhesion reaction of cultured rabbit aortic endothelial cells (RAEC) with monocytes(MC) in vitro. METHODS: The assay was based on the measurement of the myeloperoxidase achvity in monocytes that had been adhered to RAEC layers. RESULTS: At concentration from 5 to 50()U.ml-1,TNF was shown to induce adhesion reaction of cultured RAEC with MC. The effect of TNF reached maximum (81 .98%) at 250U·ml-l. After lh incubation of RAEC, addition of TNF significantly stimulated the adhesion reactin of RAEC with MC(66.8%).After 2h incubation the effect reached maximum(77.5%),then the effect slightly decreased. At concentration from 20 to 2000mg·L-１, chitosan was found to antagonize induced adhesion reaction. Its maximal rate of inhibition was 22.99% at 2000mg.L-1. CONCLUSION: Chitosan may decrease the TNF induced adhesion reaction of RAEC with MC.