摘要
以羧甲基纤维素钠（CMC）为底物评价纤维素酶的活性，研究了不同条件下静磁场对酶的活性及构象的影响。9℃，pH4．0条件下磁化，其活性及荧光光谱均不见明显变化；但若改变反应时的pH，可使活性增加达16.4％；并发现磁处理后的酶有滞后效应及可逆性。较高温度下，磁化处理酶液，其活性及构橡均发生不同程度的变化，且对其抑制动力学表现为混合型抑制模式；其构象变化也基本上随失活的增加荧光强度增大。
Cellulase actlvity is measured on sodium carboxymethyl -cellulose substrate by Dinitrosalicylic acid(DNS) method. No obvious effect is observed when magnetic field on cellulase at 9℃,pH4.0. Once change the reaction pH, the activity will be increased by 16. 4%,the delaying and reversible effect is also observed- At 60℃, the effect on the activity and conformation changes at different extent. A Lineweaver-Burk plot of the reaction shows the typical pattern of mixed-type inhibition. It seems that the intensity of the fluorescence increases with the decrease of the activity.
基金
国家自然科学基金
