白僵菌液体制剂的毒力研究

The Study on the Virulence of a Liquid Preparation of Beauveria Bassiana

探讨了生理食盐、甘油对白僵菌液体制剂的保护作用 ,比较了球孢白僵菌粉剂及液体制剂对马尾松毛虫的毒力的差异 ,其结果显示液体保护剂以 2 %甘油较为理想 ,可在 2 5℃下保存 45～ 6 0d ,液体表面不出现菌膜 ,萌发率未明显下降 ,而生理食盐不能起到有效的保护作用 ,以 2 %甘油作为保护剂的液体白僵菌和麦麸菌粉对松毛虫的致病速度、总僵死率无显著差异 ,均在 9d时出现僵死 ,12d时达僵死高峰 。

Preservative effects of two preservatives on the liquid preparation of Beauveria bassiana were also tested. The virulence of liquid preparation of B.bassiana to Dendrolimus Punctatus Walker was explored. The experiment shows that 2% glycerine (an ideal preservatative) preserves surface of liquid from appearing mycelial membrance, and maintains the high spore germination of B.bassiana for 45～60 days under 25℃ while physiological slat has no effective protection on the liquid preparation. The liquid preparation is the same as the powder of B.bassiana in both the pathogenetic rate and general death of Dendrolimus Punctatus Walker. The death of the insect by respectively spraying of two preparations all begins to appear in the 9 th day, and then reaches peak in the 12 th day. The result further illustrates the possibility of applying the liquid preparation of B.bassiana.