茶多酚中儿茶素类的HPLC分析方法学考察

Study on the Method of HPLC Analysis of Catechins in Tea Polyphenols

本文建立了一种非梯度洗脱的儿茶素HPLC定性定量分析方法 ,同时对茶多酚中的四种主要儿茶素(EC ,EGCG ,ECG ,EGC)考察了其方法学。得到最佳色谱条件为 :ODS (5 μm ,4 6× 2 5 0mm)柱 ,以重蒸水∶乙睛∶乙酸乙酯 =86∶12∶2 (v v v)为流动相 (浓硫酸调pH值至 3 0～ 4 0 ) ,流速为 1 0ml min ,UV检测波长 2 80nm。

A kind of analytical method by high performance liquid chromatography(HPLC) for tea catechins with ingradient elution system was put forward In the meantime,the method was investigated in the methodological aspect through separating and determining the main four catechins (EC,ECG,EGC,EGCG) of tea polyphenols The optimum chromatographic conditions were as follows:ODS column (5 μm,4 6×250 mm),water/acetonitrile/ethylacetate (v/v/v,8 6∶12∶2) as mobile phase (add H 2SO 4 to adjust pH 3 0～4 0),UV detector (280 nm),flow rate:1 0 ml/min Moreover,the precision and stability of the method was discussed