我国SEO型汉坦病毒L基因核苷酸序列测定研究

Study on L Gene Necleotide Sequence of Hantaan SEO Type Virus in China

目的 为各基因型或亚型病毒生物学表型研究打基础 ,对疫苗的选择使用提供科学依据。方法 将获得SEO型病毒分离、MCABS分型RT -PCR扩增及核苷酸序列测定。结果 首次测出我国SEO型病毒部分L基因核苷酸序列 ,并与 76 - 118、C4、L99、Seoul株的核苷酸及氨基酸同源性进行比较 ,分别为 70 8%、72 %、95 3%、95 9%及 75 6 %、75 1%、97 7%、97 3 %。结论 病毒基因组间的重排不是造成发病高峰的原因。

Objective To lay basis for studing and to provide scientific basis for selecting vaccine SEO viral genes.Methods Viral isolation MCABS typing and RT-PCR were used to determinate a SEO strain.Nucleotide sequence of its L gene was detected.Results The nucleotide sequence of partial L region of SEO virus was acquired for the first time in China.Compared with 76-118 C4 L99 and Seoul strain,the homologies of nucleotide sequence were 70 8%?72%?95 3%?95 9%,respectively,amino acid sequences were 75 6%?75 1%?97 7%?97 3% repectively.Conclusion The high epidemics of HFRS wasn't because of recombinant of viral genes.