燃煤型砷中毒皮肤病变中MDA、4-HNE的表达

Expression of MDA、4-HNE in Skin Pathological Chnage Caused by Arsenism of Coal-Burning

目的 探讨燃煤型砷中毒皮肤病变的机理。方法 用免疫组织化学法检测燃煤型砷中毒患者皮肤病变中脂质过氧化代谢产物丙二醛 (MDA)、4-羟壬烯醛 (4-HNE)的表达情况 :同时测定患者血中SOD、GSH -Px活性和MDA含量。结果 砷中毒患者皮肤组中MDA、4-HNE的表达强度和密度随患者皮肤病变程度的加重而逐渐加强 ,MDA、4-HNE在癌变组 (D组 )、癌前病变组 (C组 )、过度角化组 (B组 )中普遍强表达并高于一般增生病变组 (A组 )和正常组 ,各病变组间也有不同程度的差异 (P <0 0 5 ,P <0 0 1) ;而血细胞SOD、GSH -Px活力、血浆MDA含量与正常人群比较差异均有统计学意义 ,但病变组间无明显差别。结论 免疫组织化学法检测MDA、4-HNE可敏感而特异地反映砷致机体氧化损伤程度 ,有助于砷中毒的早期发现及病变进展的动态观察。

Objective To the mechanism of skin pathologic change caused by arsenism resulting from coal buring.Methods The expressions of malondialdelyde(MDA) and 4-HNE in the skins were analysed by EnVision TM +System immuno histochemistry methods.The activity of superoxide dismutase (SOD),glutathione peroxidase(GSH-Px) and the content of malondialdelyde(MDA) in blood of patients were measured and analysed.Results The positive cell density and the positive percentages of MDA and 4-HNE gradually strengthened along with the patient's skin pathological changes worsened.The above mentioned bio markers in hyperkeratosis group(B group),pre carcinoma group(C group) and carinoma group(D group) were all over the normal group or general pathological changes group (A group)( P<0 05,P <0 01),and among the every pathological changes groups existing significance to varying degrees( P<0 05,P <0 01).Compared with the control,the SOD and GSH-Px activity were lower( P <0 05),while the MDA content were higher( P <0 05) in endemic subjects.Conclusions It suggested that the immuno histochemistry assay of MDA and 4-HNE can be used as a sensitive and specialized method to reflect oxidative damage,and may help early finding and monitoring of arseniasis.