锰对镉致孕鼠毒性的拮抗作用研究

Study on Antagonism of Manganese to Cadmium Toxicity in Pregnant Rats

目的 为探讨锰对镉致孕鼠毒性的拮抗作用。方法 把Wistar孕鼠随机分为镉中毒组、补充锰组和正常对照组 ,在大鼠妊娠第 7、9、11天时称体重并给前两组孕鼠注射氯化镉使其中毒 ,于妊娠第 2 0天处死时 ,取心脏血 ,观察胎鼠发育情况 ,同时取孕鼠肝和肾脏制作组织切片 ,分别在光学显微镜和电子显微镜下观察组织、细胞形态 ,并检测血液、肝和肾脏中镉和锰含量。结果 补充锰组孕鼠体重增长率、正常活胎的比例及平均体重均显著大于镉中毒组 :而畸胎和吸收胎发生率则明显降低 (P <0 0 1) ;补充锰组孕鼠肝、肾组织结构接近正常 ;血液、肝和肾脏中镉含量显著低于镉中毒组 (P <0 0 1) ,而锰含量变化不大。结论 适量补充锰对镉中毒所致孕鼠生长发育障碍和肝、肾组织细胞损伤具有拮抗作用。

Objective To explore the antagonism of manganese to toxicity of cadmium(Cd) in pregnant rats.Methods Pregnant Wistar rats were divided into three groups:Cd-poisoning group,manganese supplement group and control group.All pregnant rats were weighed on the 7th,9th,11th day,and those in the first two groups were given injection with cadmium chloride for intoxication.On the 20th day during gestational period all rats were anaesthetized to draw blood for a test,killed to observe growth condition of fetuses.Rats' liver and kidney were extracted to make tissue sections for optical and electron microscopic examination.The contents of cadmium and manganese in blood,liver and kidney were determined.Results The growth rate of rats,the proportion of living fetuses and their average weight in the manganese supplement group increased significantly compared with those of Cd - intoxication group,while the incidence of malformed fetuses and absorbed embryos decreased greatly ( P <0 01).The structures of liver and kidney cells of rats in manganese supplement group were close to normal.The content of cadmium in blood,liver and kidney of manganese supplement group was lower than that of the Cd-poisoning group significantty( P <0.01),but the content of manganese has not changed obviously.Conclusion The adequate manganese supplement can antagonize the development disorder and cell damage in liver and kidney of rats induced by cadmium.