家蝇对DDVP抗性的生物化学标记的探索

Biochemical label of DDVP-resistant kouse fly

目的 探讨家蝇对DDVP抗性与乙酰胆碱酯酶敏感度和羧酸酶活性的关系 ,并探索家蝇对DDVP抗性生物化学标记 ,应用于家蝇抗药性的早期检测。方法 用乙酰胆碱酯酶对DDVP的敏感度作为家蝇抗性的生物化学标记检测了北京4个城区家蝇种群中具有不敏感乙酰胆碱酯酶个体的频率及其季节变化。结果 丰台区家蝇种群中到 10月份具有不敏感乙酰胆碱酯酶个体的频率明显高于其它 3个城区 ;海淀区具有不敏感乙酰胆碱酯酶的个体频率最低 ,一年中的变化也明显低于丰台区。西城区家蝇种群与海淀区比较接近 ,而朝阳区的种群则更接近于丰台区种群。结论 用药剂对CarE的抑制能力和AchE的敏感度作为家蝇对DDVP抗性的生物化学标记监测家蝇抗药性的变化是可行的。

Objective By comparing the sensitivity and activity of Acetylcholinesterase and Carboxylesterases to DDVP between sensitive strain and resistant house fly, a linear relationship among the level of DDVP-resistance, DDVP-sensitivity to Acetylcholinesterase and the level of DDVP-control to Carboxylesterases, and a non linear relationship among the level of DDVP-resistance, the activity of Acetylcholinesterase and Carboxylesterases were found.Methods The DDVP-sensitivity of Acetylcholinesterase was used as the biochemical label of house fly resistance. The frequency and change among seasons of non-Acetylcholinesterase sensitive strains of house fly were detected among 4 districts in Beijing.Results The frequency of non-Acetylcholinesterase sensitive strains of house fly in Fengtai district is significantly higher than the other 3 districts until October. The frequency of non-Acetylcholinesterase sensitive strains in Haidian district is the lowest and the level of changes is distinctively lower than that in Fengtai. The strains of house fly in Xicheng district are similar to Haidian and the strains in Chaoyang are similar to Fengtai.Conclusion It is possible to use chemicals to controt CarE and AchE sensitivity as the biochemical label of resistant house fly to detect the change of house fly resistance.