金属硫蛋白ⅡA过表达对乳腺癌细胞耐药性的影响

Effects of MT Overexpression on Drug Resisatnce in Breast Cancer Cells

目的 研究金属硫蛋白 ( Metallothionein,MT)基因过表达对乳腺癌细胞耐药性的影响。方法 表达质粒 p BAc Neo MT A转染 MCF- 7/Adr细胞 ,用 SRB方法检测了 MT过表达细胞对顺铂的耐药性的变化。结果 与对照细胞相比 ,p BAc Neo MT A转染的细胞对顺铂的耐受性提高 ,其 LD50 由约 1 2 u M增加到大约 30 u M。结论

Objective To study the effects of MT(Metallothionein)overexpression on drug resistance in breast cancer cells.Methods Expression plasmid pBAcNeoMT ⅡA was transfected into breast cancer cell lineMCF 7/Adr cells.The drug resistance of the cells to cisplatin were detected by SRB experiment.Results Compared to the control cells,MCF 7/Adr cells transfected with MTⅡA cDNA showed enhanced resistance to cisplatin,The LD 50 increases from about 12 uM to approximately 30uM.Conclusion Overexpression of MT can increase resistance to cisplatin in breast cancer cells.