耳针对血管性痴呆大鼠记忆及海马凋亡相关蛋白表达的影响

Effects of Auricular Needling on Memory and Expression of Apoptosis-Related Proteins in Hippocampus in Rats of Vascular Dementia

目的 :探讨耳针对血管性痴呆大鼠 (VD)学习记忆障碍的改善与bcl 2、bax凋亡相关蛋白表达的关系。方法 :采用 4 血管阻断制备VD大鼠模型 ,针刺脑、肾耳穴后 ,作免疫组化、行为学测试及图像分析。结果 :耳针治疗后VD大鼠海马CA1区bcl 2、bax蛋白表达增加 ,特别是bcl 2明显增加 ,与学习记忆成绩呈正相关。结论 :耳针改善VD大鼠学习记忆 ,可能是通过针刺调控细胞凋亡 。

Purpose To approach to relation of improvement of learning and memory with expression of apoptosis related proteins bcl 2 and bax in the rat of vascular dementia(VD).Methods VD rat model was prepared by occlusion of four blood vessels and after acupuncture at auricular points Nao and Shen,immunohistochemical analysis,behavioral observation and image pattern analysis were made.Results After treatment of auricular needling,expressions of bcl 2 and bax proteins in CA 1 region of hippocampus increased,especially,bcl 2 had obvious increase,which was positively correlated with learning and memory.Conclusion Auricular needling improves learning and memory in rats of VD possibly through control of apoptosis of cells to protect neurons of hippocumpus.