坐骨神经损伤后生长相关蛋白表达的免疫组化研究

THE IMMUNOHISTOCHEMICAL STUDY OF GAP-43 EXPRESSION IN LESIONED SCIATIC NERVE

目的 :探讨周围神经在损伤后远侧端生长相关蛋白 (GAP- 43)的表达。方法 :切除 SD大鼠一侧坐骨神经 5 m m,造成坐骨神经缺损伤模型。两周后用免疫组化法检测损伤远侧端神经组织中 GAP- 43的表达。结果 :损伤神经远侧端雪旺细胞表面可见棕色阳性产物 ,而正常神经组织中未见阳性反应产物。结论 :损伤神经远侧端雪旺细胞中可见 GAP- 43表达 ,在正常神经组织中 GAP- 43低表达或无表达。

Objective:To study the expression of Growth -Associated-Protein-43(GAP-43) in the distal stump of rat sciatic nerve defect.Methods:Two weeks after rat sciatic nerve injury ,we investigated the expression of GAP-43 in the distal stump and normal nerve with immunohistochemical staining.Results: Positive reactions of GAP-43 were found on the surface of Schwann cell in distal stump while there is no expression in normal sciatic nerve. Conclution:GAP-43 localizes in the Schwann cell in distal stump after sciatic nerve injury ,but it can not be found in normal nerve.