全血化学发光法测定镉对鼠中性粒细胞吞噬功能的影响

Effect of cadmium on the phagocytic function of polymorphonuclear leukocyte in mice tested by chemiluminescence of whole blood

采用全血化学发光分析法和硫代苯巴比妥酸 (TBA)检测方法研究氯化镉对中性粒细胞 (PMN)的免疫毒性和对肝脂质过氧化作用的影响。结果表明 ,在体外 ,0 1～ 10mmol L的氯化镉可增加大鼠全血化学发光强度 ,而 10 0 0mmol L的氯化镉则抑制全血化学发光。小鼠尾静脉注射氯化镉 2h之后 ,1和 10 0 μg kg剂量组能显著诱导全血化学发光 ,而 5 0 0和 10 0 0 μg kg剂量组则对全血化学发光有抑制作用 ;1μg kg剂量的低浓度镉诱导肝脂质过氧化作用。结果提示 ,低浓度的镉对PMN具有免疫促进作用 ,高浓度的镉则对PMN具有细胞毒性。可能低浓度的镉能促进吞噬细胞的吞噬能力 ,吞噬作用使机体产生过量的活性氧 ,从而加剧了活性氧对组织细胞的脂质过氧化作用 ,最终造成机体的氧化损伤。

The effect of cadmium chloride on the immunological toxicity of polymorphonuclear leukocyte (PMN) and on lipid peroxidation in liver were measured by using the chemiluminesence of whole blood and thiobarbituric acid (TBA) method.The results showed that 0 1 and 10 mmol/L of cadmium chloride could accelerate the chemiluminesence of whole blood in vitro,but 1000 mmol/L of cadmium chloride inhibit the chemiluminesence.Two hours after the injection of cadmium chloride through caudal vein, chemiluminesence of whole blood was induced obviously at the dose of cadmium 1 and 100μg/kg BW in vivo,but the chemiluminesence was inhibited at the dose of 500 and 1000μg/kg BW. At the dose of 1μg/kg BW, the peroxidation of liver lipids was induced. The results suggested that cadmium at low concentration accelerated the immunity of PMN, while high concentration was toxic to PMN, cadmium at low concentration could accelerate the phagocytic function of phagocytes. Phagocytosis brings about excessive active oxygen in organism and intensifies the action of active oxygen on the lipid peroxidation in tissue cells,at last,results in organic oxidation injury.