卡托普利对大鼠自体移植静脉内膜增生的影响

Effect of Captopril on the Intimal Hyperplasia of Autografted Vein of Rat

目的 :观察卡托普利 (captopril,CAP)对移植静脉内膜增生的影响 ,探讨其作用机理。 方法 :建立大鼠颈总静脉移植于颈总动脉模型。分为CAP组及对照组各 10只。CAP组于术前 7d开始用CAP灌胃 ( 10 0mg·kg-1·d-1) ,对照组用等容量生理盐水灌胃。二组均灌胃到术后 2周。采用HE、弹力纤维VG染色 ,利用计算机图像分析仪计算内膜面积 /中膜面积比率 (I/M )来表示内膜增生程度 ;测定给药前、给药后 7d、给药 2周时动脉血清NO含量。结果 :CAP组I/M为 ( 9.73± 0 .67) % ,对照组为 ( 19.0 1± 0 .70 ) % ,实验组显著低于对照组 (P <0 .0 1)。给药7d即可引起动脉血清中NO升高 ,2周时升高更加明显。结论 :卡托普利通过提高大鼠动脉血中NO含量抑制移植静脉内膜增生。

Objective: Our purposes were to observe the effect of captopril on the intimal hyperplasia of autografted vein and to discuss the mechanism. Methods: Common jugular vein was interplanted to the common carotid artery of Wistar rats to establish the animal models. The rats were devided into captopril (CAP) group and control group, with 10 rats in each group. The CAP group was given CAP by gavage 7 days before operation (100 mg·kg -1 ·d -1 ) , normal saline of the same volume was given to the control group. Drugs were given to each group until 2 weeks after operation. HE and elastin fiber VG staining were adapted. To show the hyperplastic degree of intimal, the ratio of intimal area/media area (I/M) was calculated with computer assisted figmeanalyzer. Nitric oxide (NO) content in the arterial blood serum was measured seperately before giving CAP 7 days and 2 weeks after giving CAP. Results: The I/M in CAP group was (9.73±0.67)%,and in the control group it was (19.01±0.70)%. The I/M value of CAP group was lower than that of the control group significantly (P<0.01). The NO content in the arterial blood increased 7 days after CAP had given , and reached to a higher level 2 weeks later. Conclusion: Intimal hyperplasia of graft vein can be inhibited by captopril through enhancing the content of NO in rat arterial blood.