止喘胶囊对实验性哮喘动物模型气道重建的影响

Study on Effect of Zhichuan Capsule on Airway Remodeling in Experimental Animal Model of Asthma

目的 :阐明止喘胶囊 (补肾健脾法 )治疗哮喘对气道重建的影响及其可能的机制。方法 :将50只SD大鼠随机分为正常对照组、模型组、止喘胶囊大剂量组、止喘胶囊小剂量组、必可酮组 ,每组 10只。通过长期吸入卵白蛋白建立慢性哮喘模型 ,肺组织切片分别进行胶原染色、纤维连接蛋白 (Fn)免疫组织化学染色 ,借助计算机图像辅助分析系统分析气道壁胶原、Fn含量、气道腔面积变化 ,显微测量气道腔内径变化。结果 :模型组气道壁胶原、Fn含量明显高于正常对照组 (P <0 0 5,P <0 0 1) ,气道内 /外径比值、气道内腔面积 /总横截面积比值明显低于正常对照组 (均P <0 0 1)。止喘胶囊大剂量组气道壁胶原、Fn含量明显低于模型组 (P <0 0 5,P <0 0 1) ;气道内 /外径比值、气道内腔面积 /总横截面积比值明显高于模型组 (P <0 0 5) ;且与正常对照组相似 (P >0 0 5)。止喘胶囊小剂量组的气道壁胶原含量、气道内 /外径比值和气道内腔面积 /总横截面积比值虽与模型组无差异 (P >0 0 5) ,但其Fn含量明显低于模型组 (P <0 0 1)。结论 :止喘胶囊 (补肾健脾法 )可能通过减少气道壁胶原和Fn沉积 ,抑制慢性哮喘的气道重建 ,有利于对慢性哮喘的治疗和预防。

Objective: To elucidate the effect and possible mechanism of Zhichuan Capsule (ZCC), a Chinese herbal preparation for reinforcing Kidney and invigorating Spleen, on airway remodeling when it was used in treating asthma. Methods: Fifty SD rats were randomly divided into five groups: the normal control group, the model group, the high-dosage ZCC group, the low-dosage ZCC group and the Becotide (Beclomethasone Dipropionate) group, 10 rats in each group. The chronic asthma model was established by repeated inhalation of ovalbumin. The changes of collagen and fibronectin (Fn) content in airway wall, inner and outer diameter as well as area of respiratory tract cavity in lung slices were measured by computerized image analysis system. Results: The wall contents of collagen and Fn in airway were higher (P<0.05 and P<0.01), while the ratio of inner diameter/outer diameter (ID/OD) and ratio of area of airway cavity/total area of airway (CA/TA) were significantly lower (P<0.01) in the model animals than those in the normal controls. As compared with the model group, collagen and Fn contents were lower, ID/OD and CA/TA ratio were significantly higher in high-dosage ZCC group, close to normal range. In the low-dosage ZCC group, although collagen content, ID/OD and CA/TA were not different to those in the model group, but the content of Fn was significantly lower (P<0.01). Conclusion: ZCC could inhibit the remodeling of airway in chronic asthma by way of reducing the precipitation of collagen and Fn, thus help the prevention and treatment of chronic bronchial asthma.