心和颗粒剂对高脂饮食大鼠血管内皮损伤及内皮表达内皮素、粘附分子的影响

Effect of Xinhe Granule on Vascular Endothelial Damage and Endothelial Expressed Endothelin and Intercellular Adhesion Molecule-1 in Rats Fed with High Lipid Diet

目的 :研究心和颗粒剂对高脂饮食大鼠血管内皮损伤的保护作用。方法 :通过高脂饮食造成大鼠血管内皮损伤 ,灌药组分为高剂量心和组、低剂量心和组、丹参滴丸组 ,同时设空白组、高脂对照组 ,利用内皮细胞铺片技术和免疫组化技术定量检测各组内皮细胞损伤程度及合成分泌内皮素 (ET 1)、细胞间粘附分子 1(ICAM 1)的阳性细胞数。结果 :各组血管内皮损伤程度及内皮分泌ICAM 1、ET 1比较 ,高脂对照组>丹参滴丸组 >低剂量心和组 >高剂量心和组 >空白组。心和颗粒剂无明显降低血脂作用。结论 :心和颗粒剂能抑制高脂血症大鼠血管内皮表达ICAM 1、ET 1,对血管内皮有保护作用。

Objective: To study the protective effect of Xinhe granule (XHG) on vascular endothelial damage in rats fed with high lipid diet. Methods: Model of vascular endothelial damage was formed by feeding high lipid diet in rats. The model animal were divided into high dosage XHG group, low dosage XHG group, composite Salvia dripping pellet group and model control group, and a blank (normal) control group was also set up. The degree of endothelial damage and positive cell count of synthesizing and secreting endothelin (ET-1) and intercellular adhesion molecule-1 (ICAM-1) were determined by endothelial cell spreading technique and immunohistochemical technique quantitatively. Results: Comparison of the degree on vascular endothelial damage and endothelial secreting ICAM-1 and ET-1 showed model control group > composite Salvia dripping pellet group > low dosage XHG group > high dosage XHG group > blank control group. XHG did not show obvious lowering action on blood lipid. Conclusion: XHG could inhibit the endothelial expressed ICAM-1 and ET-1 in hyperlipidemia rats, thus displaying the protective effect on vascular endothelium.