肝硬化患者外周血中TGFβ_1和ET-1的表达

TGFβ_1 and ET-1 Expression in the Peripheral Blood of Patients with Cirrhosis

目的 研究肝硬化患者外周血中转化生长因子 β1 (TGFβ1 )及血浆内皮素 - 1(ET- 1)的表达 ,并探讨其与肝脏贮备功能和食管静脉曲张的关系。方法 采用标准生物学方法即 TGFβ1 对水貂肺上皮细胞生长抑制的方法及放免法分别检测 72例肝硬化患者及 2 4例正常人血清 TGFβ1 及血浆 ET- 1水平。结果 肝硬化组血清TGFβ1 (11.77± 1.32 ng/ m l)及血浆 ET- 1(78.37± 17.5 4pg/ ml)水平明显高于正常对照组 (P<0 .0 5 ) ;ET- 1水平与肝功能及食道静脉曲张程度相关分析显示 ET- 1水平越高则肝脏贮备功能越差 (r=0 .94) ,食道静脉曲张程度越重 (r=0 .87) ;TGFβ1 含量与肝脏贮备功能及食道静脉曲张程度无关 (r=0 .11及 0 .0 3)。结论 TGFβ1 和 ET- 1可能是参与肝硬化病理生理过程的重要神经肽。ET- 1可作为反映肝硬化程度、判断预后的参考指标。

Objective The aims of this study were to determine ① the variation of blood TGFβ 1 and ET 1 in patients with liver cirrhosis; ② the relationship of the blood TGFβ 1 and ET 1 with Child degree of liver function and the severity of esophageal varices. Methods Peripheral blood samples were obtained from 72 patients and 24 normal controls.The blood levels of TGFβ 1 and ET 1 were measured by using a standard bioassay and the radioimmunoassay.Results TGFβ 1 level (11.77±1.32 ng/ml)and ET 1 level(78.37±17.54 pg/ml) were significantly higher in the patients than those in controls( P <0.05). The increase of the ET 1 in the patients is closely associated with Child degree of liver function ( r =0.94) and the severity of esophageal varices( r =0.87). TGFβ 1 concentration is not associated with Child degree of liver function ( r =0.11) and the severity of esophageal varices ( r =0.03) . Conclusion TGFβ 1 and ET 1 may play an important role in the pathophysiologic process of cirrhosis. ET 1 concentration may reflect portal hypertension development.