O_3衰老小鼠模型的DNA损伤研究

Study on DNA Oxidative Damage of O_3 Aging Model in Mice

目的 探讨自由基致衰老的小鼠模型 DNA氧化损伤的情况。方法 通过给 2月龄小鼠吸入一定量的 O3,建立 O3衰老小鼠模型 ;然后以单细胞凝胶电泳试验检测 O3衰老小鼠模型、自然衰老小鼠和正常青年小鼠 (阴性对照组 )的脾淋巴细胞 DNA氧化损伤情况。结果 与阴性对照组相比 ,O3衰老小鼠模型的脾淋巴细胞DNA受到较显著的氧化损伤 (P<0 .0 5 ) ;其损伤程度与自然衰老小鼠相近 (P>0 .0 5 )。结论 O3衰老动物模型与自然衰老近似 ,在衰老的研究和抗衰老药物的药理实验中具有一定应用价值。

Objective This study was aimed to detect the animals' DNA oxidative damage. Methods A free radical mouse aging model was established by putting the mouse in a device for inhaling quantities of ozone (O 3).Single cell gel electrophoresis (SCGE) was used for assessing the splenolymphocytes' DNA damage. Results The appearance rate of comet and the length grade of DNA migration for the young control group ( P <0.05). No significant difference in the degree of DNA damage was observed between the O 3 model group and the natural aging group( P >0.05).Conclusion The DNA damage of O 3 animal model is consistent with that of natural aging animal;this animal model DNA damage of O 3 animal model is consistent with that of natural aging animal; this animal model and the method of SCGE are useful for future researches on aging and antiaging medicine.