胎鼠血清胰岛素样生长因子水平改变与宫内生长迟缓的关系研究

The Relationships of the Serum Concentrations of Insulin-like Growth Factors in Fetal Rats with Intrauterine Growth Retardation

目的 观察胎鼠血清胰岛素样生长因子 和 (IGF- 和 IGF- )的变化 ,探讨 IGFs与胎儿宫内发育迟缓 (IUGR)发生的关系。方法 2 1只 SD孕鼠被随机分为对照组 9只 ,实验组 (IUGR) 12只。实验组于第14天行双侧子宫动、静脉钳夹 2 0分钟以建立胎鼠 IUGR模型。对照组仅行开腹和关腹术。比较两组胎鼠血清 IGFs的浓度及体重、身长和肝、肺、脑及胎盘的重量。结果 实验组血清 IGF- 、 浓度分别为 117.92± 2 6 .5 8ng/ ml和2 33.19± 33.35 ng/ ml,明显低于对照组 (分别为 2 34 .43± 70 .6 5 ng/ m l和 397.74± 2 3.6 9ng/ m l) (P均 <0 .0 1) ,其体重、身长及肝、肺、脑、胎盘组织的重量亦明显低于对照组 (P均 <0 .0 5 )。结论 胎鼠血 IGF- 和 IGF- 水平的降低是 IU GR发生的内在因素之一 ,IGFs在胎儿生长发育中有重要的作用。

Objective To observe the alterations of serum insulin like growth factor Ⅰ, Ⅱ(IGF Ⅰ, IGF Ⅱ)in rat fetus and to investigate the relationship of serum IGFs with intrauterine growth retardation (IUGR). Methods 21 pregnant SD rats were randomly divided into control group ( n =9) and experimental group (IUGR group, n =12). On day 14 of gestation, the bilateral uterine arteries and veins of experimental group were clamped for 20 minutes to build the rat IUGR models and sham surgeries were performed on control group. Fetal serum concentrations of IGFs, birth weight, the length and weight of fetal liver, lung, brain and placenta of all rats in the two groups were measured and compared. Results The serum concentrations of IGF Ⅰ, Ⅱ of IUGR group were 117.92±26.58ng/ml and 223.19±33.35ng/ml, respectively, which were significantly lower than those of control group (234.43±70.65ng/ml and 397.74±23.69ng/ml, respectively, P <0.01). Likewise,birth weight, the length and weight of liver, lung, brain and placenta in IUGR group were significantly lower than those in control group ( P <0.05). Conclusion The decrease of fetal serum concentrations of IGF Ⅰ, Ⅱ may be one of the internal factors contributing to fetal IUGR,and IGFs may play an important role in fetal growth.