少突胶质细胞对PC12细胞JAK2基因表达的影响及其意义

Effects and significance of oligodendrocyte and its cell debris on the expresstion of JAK2 in PC12 cells

目的 探讨少突胶质细胞对PC1 2细胞JAK2基因表达的影响及其意义。方法 用分离培养的少突胶质细胞及其细胞碎片分别作用于培养的PC1 2细胞 ,于不同作用时相点在观察PC1 2细胞突起变化的同时检测PC1 2细胞内JAK2mRNA的表达及JAK2蛋白的磷酸化。结果 在给予少突胶质细胞及其细胞碎片处理后 1 0min ,JAK2mRNA表达较对照组有显著增高 (P <0 .0 5) ,但处理 1 2h后 ,其表达虽较处理 1 0min及 4h有显著下降 ,但仍显著高于对照组 (P <0 .0 5)。对照组JAK2蛋白的活性较低 ,但少突胶质细胞及其细胞碎片加入后 ,JAK2蛋白很快便被磷酸化而激活 ,到处理后 1 2h ,激活的蛋白虽较处理 1 0min有显著下降 ,但仍显著高于正常对照组 (P <0 .0 5)。少突胶质细胞及其细胞碎片对其影响差别不显著 (P >0 .0 5)。结论 少突胶质细胞作用于PC1 2细胞时可调节PC1 2细胞JAK2的表达与激活 ,这种表达与激活在一定程度上可能介导少突胶质细胞对PC1 2细胞突起生长的影响。

Objective To investigate the effects of oligodendrocyte and its debris on the expression of JAK2. Methods PC12 cells were cultured with oligodendrocytes and its debris respectively. mRNA expression and protein phosphorylation of JAK2 in PC12 cells were examined at different time after treatment. Results Both the level of mRNA and phosphorylated protein of JAK2 increased at 10 min and 4 h and then went down at 12 h (still higher than the control) after treated with oligodendrocyte or its debris. No significant difference of the effects was found between dendrocyte and it's debris. Conclusion Oligodendrocyte might participate in the regulation of both the expression of JAK2 and the activation of JAK2 protein which is thought to be involved in the inhibition growth of neurite.