肝脏缺血再灌注损伤时大鼠血清谷胱甘肽及其相关酶活性的变化

Changes of serum reduced glutathione and related enzymes in hepatic ischemic reperfusion injury in rats

目的 还原型谷胱甘肽 (GSH)是体内重要的氧自由基的清除剂。本实验意在研究缺血肝脏复流后血清谷胱甘肽及其相关酶水平变化 ,及L 精氨酸对其水平的影响。方法 雄性SD大鼠 90只随机分为 3组 :左中肝叶先行缺血 70min ,后恢复血流 ,于复流前静脉给予生理盐水 (损伤组 ) ,或复流前静脉给与L 精氨酸 ( 2 0 0mg/kg) (处理组 ) ;假手术组只给与暴露左中肝叶之肝蒂。各组动物分别在复流后 0、1、3、6、1 2h取血清标本 ,用以检测丙氨酸氨基转移酶 (ALT)、MDA、还原型谷胱甘肽 (GSH)、谷胱甘肽过氧化物酶 (GSH PX)和谷胱甘肽 S转移酶 (GSH ST)。并取肝组织标本做光镜和电镜观察 ,了解组织病变特点。结果 损伤组血清ALT、MDA、GSH ST和GSH PX含量较假手术组有明显的提高 (P <0 .0 5) ,而在复流后 3h血清GSH水平明显降低 (P <0 .0 5)。给与L 精氨酸处理可以明显减轻这种变化 (P <0 .0 5)。结论 L 精氨酸对大鼠肝脏缺血再灌注损伤的保护作用可能与上调还原型谷胱甘肽水平有关。血清GSH PT、GSH ST活性受到肝细胞损伤后释放增加的影响 ,与肝脏损伤程度有关。

Objective The release of free radical (FR) is associated with hepatic ischemic reperfusion (IR) injury. In this study, the effects of nitric oxide on serum reduced glutathione (GSH) and related enzymes were investigated. Methods A total of ninety male SD rats were devided into three groups: sham operation (GroupⅠ) and 70 min ischemia followed by reperfusion with being given L arginine (200 mg/kg) (GroupⅢ) or saline (GroupⅡ) intravenously just before the reflow. Rats were sacrificed at 0,1,3,6 and 12 h after reperfusion. Serum alanine aminotransferase (ALT), methylenedioxyamphetamine (MDA), reduced glutathione (GSH), glutathione peroxidase (GSH PT) and glutathione sulfurtransferase (GSH ST) were determined. The morphologic change of the liver was studied. Results Serum AST, MDA, GST PX and GSH ST increased significantly in GroupⅡ while GSH decreased significantly from 3 h after reperfusion. The above changes were reversed by the NO donor, L arginine significantly. Conclusion These results demonstrate the changes of serum GSH,GSH PX and GSH ST in the hepatic IR injury and reflect the extent of tissue injury. These may correlate with the protective role of L arginine in the hepatic IR injury.