高温高湿环境犬肢体火器伤后HSP70含量变化的实验研究

Dynamic change of HSP70 after gunshot wound in canine limbs in hot and humid environment

目的探讨高温高湿环境下肢体火器伤后热休克蛋白７０（ＨＳＰ７０）含量变化的规律。方法将１８只犬随机分为高温 高湿组、常温常湿组、热适应组，分别在致伤前及火器伤后ｌ、３、４、６、８、１０、１４、１８、２４ｈ检测外周血淋巴细胞和伤道骨骼 肌中ＨＳＰ７０含量。结果淋巴细胞中ＨＳＰ７０含量在高温高湿组的两个高峰分别为伤后４ｈ和ｌ４～ｌ８ｈ；热适应组３ｈ达 到高峰，持续至６ｈ；常温常湿组６ｈ达到高峰。常温常湿组伤道骨骼肌ＨＳＰ７０含量在伤后ｌ４～１８ｈ也出现一个增幅不 明显的高峰期。结论 高温高湿环境下肢体火器伤后ＨＳＰ７０含量的变化有其独特的规律。

Objective: To investigate the dynamic change of heat shock protein 70 (HSP70) after gunshot wound in the canine limbs in hot and humid environment Methods Eighteen dogs, after gunshot wound in the posterior limbs were induced, were divided into normal environment group (NE), heat acclimatization group (HA) and hot and humid environment (HHE) group. The content of HSP70 in the peripheral blood lymphocytes and in the muscular tissues in the wound tract was measured at different time points after injury. Results HSP70 in the peripheral blood lymphocytes peaked twice at 4h and 14 to 18h after injury respectively in HHE group, and there was one peak at 3 h, lasting till 6h, in HA group. The content of HSP70 reached the maximum 6h after injury in NE group. In the muscular tissues in the wound tract an inconspicuous peak of HSP70 content was observed 14 to 18 h after injury in NE group. Conclusion Differing from normal conditions, hot and humid environment may produce peculiar changes of HSP70 content in dogs after gunshot injuries.